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Effect of leaf extract of
Seabuckthorn on lipopolysaccharide induced inflammatory
response in murine macrophages.
Immunomodulation
Laboratory, Defence Institute of physiology and Allied
Sciences, Lucknow Road, Timarpur, Delhi, 110054, India.
Nitric oxide (NO)
is synthesized in large quantities by activated
inflammatory cells and has been demonstrated to be
involved in the pathogenesis of acute and chronic
inflammatory conditions. Seabuckthorn (SBT) has been used
in traditional medicine systems for the treatment of
various diseases like cardiovascular, pain relief, oral
inflammation and promotion of tissue regeneration. The
present study focuses on the effects of SBT leaf extract
on NO production induced by lipopolysaccharide (LPS) in
the murine macrophage cell line RAW 264.7. In addition,
cell viability, free radical-scavenging activity and
inducible nitric oxide synthase (iNOS) expression were
also evaluated. Seabuckthorn leaf extract significantly
inhibited the enhanced production of NO induced by LPS in
a dose dependent manner. Treatment with SBT did not reduce
cell viability at any dose used. The extract showed
significant scavenging of NO radicals released by the NO
donor. Treatment of macrophages with SBT leaf extract also
caused a significant inhibition of iNOS activation. These
observations suggest that the inhibition of net NO
production by SBT leaf extract may be due to its
scavenging activity and/or its inhibitory effects on iNOS
activation. The study suggests
that SBT leaf extract has significant anti-inflammatory
activity and has potential for the treatment of
inflammatory diseases.